DAROU PAKHSH PHARMA. CHEM . Co.

DAROU PAKHSH PHARMA.CHEM. CO.

Quality control Lab

Certificate of analysis

   Product:    Atenolol           C14H22N2O3

Test

Limits

Description

A white crystalline powder

Solubility

Very soluble in water, freely soluble in alcohol, practically insoluble in ether.

Identification

A. Melting point 152°C to 155°C.
B. Absorbance ratio 275/282nm 1.15 to 1.20.
C. Infrared spectrophotometry as same as Atenolol CRS
D. TLC RF value of test and Atenolol CRS are the same.

Appearance of 1% solution

Is clear and not more intensely colored than degree 6 of the range of reference solutions of the most appropriate color

Optical rotation

+0.10° to -0.10°

Related substances HPLC

The area of any peak apart from the principle peak is not grater than 0.25%, the sum of such area not grater than 0.5%.

Chlorides

Not more than 0.1 per cent.

Loss on drying

Not more than 0.5 per cent.

Sulphated ash

Not more than 0.1 per cent.

ASSAY

99.0 - 101.0 % w/w calculated with reference to the dried substance

This material has been prepared following the good manufacturing practice (G.M.P.) and conforms above specification & requirements of BP2000